5-Azacytidine-Induced Cardiomyocyte Differentiation of Very Small Embryonic-Like Stem Cells

Figure 2

Bone marrow- (BM-) derived very small stem cells (VSELs) retain embryonic stem cell (ESC) characteristics. (a) Rat BM-derived VSELs were obtained by flow cytometric sorting. (b) BM-VSELs were assessed for alkaline phosphatase (AKP) activity by staining for AKP. (c) Expression of Sox2, Oct-4, and Nanog in VSELs was assessed by immunostaining with Sox2-, Oct-4-, and Nanog-specific primary antibodies and a Cy3 secondary antibody.

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