Effect of Inflammation on Gingival Mesenchymal Stem/Progenitor Cells’ Proliferation and Migration through Microperforated Membranes: An In Vitro Study

Figure 2

(a) Flow cytometric analysis of the surface marker expressions CD90, CD73, and CD105 in healthy gingival tissues. (b) Flow cytometric analysis of the surface marker expressions CD90, CD73, and CD105 in inflamed gingival tissues. (c) Alizarin red staining of calcium deposits of GMSCs in osteogenic medium, Oil Red staining of oil droplets of GMSCs in adipogenic medium, and Alican blue staining of cartilage glycoprotein of GMSCs in chondrogenic medium and their respective controls. (d) Population doubling time assay with means and standard deviation of the of GMSCs from healthy and inflamed gingival tissues, with significantly shorter population doubling time in the inflamed group. (e) Graph showing colony-forming unit assay with means and standard deviation of the of GMSCs from healthy and inflamed gingival tissues.

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