circRNA Expression Profile in Dental Pulp Stem Cells during Odontogenic Differentiation

Figure 1

Identification and multiple differentiation potential of hDPSCs. (a) hDPSCs were separated from dental pulp. (b) hDPSCs were subcultured with normal media at third passage. (c–h) hDPSCs were positive for Stro-1 (18.39%), CD44 (99.99%), CD29 (97.96%), and CD90 (99.99%) and negative for CD45 (0.46%) and CD34 (0.35%). (i, j) A single cell was obtained, and single cell-derived colonies were obtained after culturing for 14 days. (k, 1) Noninduced group and induced group of odontogenic differentiation. Mineral nodes formed in the induced group were as indicated by the yellow arrow. (m, n) Noninduced group and induced group of adipogenic differentiation. Lipid droplets formed in the induced group were indicated by the yellow arrow. (o, p) Noninduced group and induced group of chondrogenic differentiation. Expressed glycosaminoglycans formed in the induced group were indicated by the yellow arrow. (q–s) In the induced group, upregulated expressions of odontogenic differentiation-related genes DSPP, DMPI, and OCN by qRT-PCR were shown, compared with the noninduced group. All samples were performed in triplicate. The data are represented as . , .

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