Three Growth Factors Induce Proliferation and Differentiation of Neural Precursor Cells In Vitro and Support Cell-Transplantation after Spinal Cord Injury In Vivo

Figure 2

Differentiation of NPCs towards the neuronal lineage in vitro. (a-c) Costaining of GFP⁺/DCX⁺ cells (blue arrows) indicating neuronal precursors. (d) Quantification of neuronal precursors after 7 days of incubation with the different growth factor concentrations/combinations in groups 2-4, expressed in proportion to (fold to) the control group (group 1). (e-g) Costaining of GFP⁺/NeuN⁺ cells (blue arrows) indicating mature neurons. (h) Quantification of mature neurons after 7 days of incubation with the different growth factor concentrations/combinations in groups 2-4, expressed in proportion to (fold to) the control group (group 1).