Immortalizing Mesenchymal Stromal Cells from Aged Donors While Keeping Their Essential Features

Figure 10

Histochemical and immunohistochemical staining of iMSC#6 and iMSC#9 after 21 days of chondrogenic induction or culturing in 20%FBS/DMEM: Safranin O staining (a–d), Masson’s Trichrome staining (e–h), and aggrecan immunostaining (i–l) (scale bar: 100 μm; magnification: 10x). Quantification is shown for each sample as percentage of stained area or intensity (optical density).