Immortalizing Mesenchymal Stromal Cells from Aged Donors While Keeping Their Essential Features

Figure 12

Oil Red O staining of iMSC#8 (a) and iMSC#9 (b). Percentage of Oil Red O-stained area for each sample is shown (scale bar: 100 μm; magnification: 10x). Adipogenesis-related gene expression after culturing in adipogenic medium is shown comparing MSC#6, T-MSC#6, and iMSC#6 (c) and comparing iMSC#6, iMSC#8, and iMSC#9 (d). APN and FABP4 expression was not detected in cells cultured in basal medium.