Immortalizing Mesenchymal Stromal Cells from Aged Donors While Keeping Their Essential Features

Figure 13

Histochemical and immunohistochemical staining of T-MSC#6 and iMSC#6 aggregates after 21 days of chondrogenic induction: Safranin O staining of T-MSC#6 (a, 20x) and iMSC#6 (b, 10x); Masson’s Trichrome staining of T-MSC#6 (c, 20x) and iMSC#6 (d, 10x); aggrecan immunostaining of T-MSC#6 (e, 20x) and iMSC#6 (f, 10x); and type II collagen immunostaining of T-MSC#6 (g, 20x) and iMSC#6 (h, 10x). Quantification is shown for each sample as percentage of stained area or intensity (optical density).