Research Article
Immortalizing Mesenchymal Stromal Cells from Aged Donors While Keeping Their Essential Features
Figure 2
Percentage of cell population reduction (1) after spinoculation (), (2) after three-day incubation with 0.5 (a), 1 (b), 2 (c), 3 (d), or 5 (e) mM VPA (), and (3) after puromycin selection () of SV40LT-transduced MSCs (f), inferred from percentage difference between absorbance measurements employing CCK-8 (measure wavelength: 450 nm; reference wavelength: 650 nm). The error bars represent the standard deviation of measurements for three different MSC cultures ().
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