Immortalizing Mesenchymal Stromal Cells from Aged Donors While Keeping Their Essential Features

<div>SV40LT and eGFP-hTERT immunostaining of iMSC#6 (a–d), iMSC#8 (e–h), iMSC#9 (i–l), and iMSC#10 (m–p). SV40LT is showed in red, eGFP-hTERT is showed in green, and Hoechst staining is showed in blue. Both nucleoli exclusion of SV40LT and nucleoli association of hTERT are pointed with white arrows. Scale bar: 50 <i>μ</i>m.</div>

Stem Cells International

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Figure 3

Figure 3: Immortalizing Mesenchymal Stromal Cells from Aged Donors While Keeping Their Essential Features 