Immortalizing Mesenchymal Stromal Cells from Aged Donors While Keeping Their Essential Features

Figure 5

Phase-contrast microscopic images of the senescence-associated ß-galactosidase activity stained iMSC#6 (a), iMSC#8 (b), iMSC#9 (c), iMSC #10 (d) and primary MSCs (e). Magnification: 10x. Percentage of positive ß-galactosidase-stained, senescent cells, for each population (f). The error bars represent the standard deviation of measurements for three passages of each iMSC line (). Significant difference was found between iMSC lines and primary MSCs ( value < 0.0001).

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