Immortalizing Mesenchymal Stromal Cells from Aged Donors While Keeping Their Essential Features

Table 3

List of primers employed for quantitative real time PCR (qPCR) analysis.

Gene	Reference sequence	Forward primer (5 ⟶ 3)	Reverse primer (5 ⟶ 3)
Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta (YWHAZ)	NM_003406.3	GATCCCCAATGCTTCACAAG	TGCTTGTTGTGACTGATCGAC
Homo sapiens runt-related transcription factor 2 (RUNX2)	NM_001024630.4	TTACTTACACCCCGCCAGTC	TATGGAGTGCTGCTGGTCTG
Homo sapiens Sp7 transcription factor (SP7)	NM_001173467.2	TCCCCTGTTGCCATGGTTAT	CCACCCATTCTTCAGGAGGT
Homo sapiens bone gamma-carboxyglutamate protein (OCN)	NM_199173.5	GGCGCTACCTGTATCAATGG	TCAGCCAACTCGTCACAGTC
Homo sapiens adiponectin, C1Q and collagen domain containing (APN)	NM_001177800.1	GGTGAGAAAGGAGATCCAGGT	TGCTGAGCGGTATACATAGGC
Homo sapiens fatty acid-binding protein 4 (FABP4)	NM_001442.2	GGATGATAAACTGGTGGTGGA	CACAGAATGTTGTAGAGTTCAATGC
Homo sapiens HRas protooncogene, GTPase (HRAS)	NM_005343.4	TGCCATCAACAACACCAAGT	ACGTCATCCGAGTCCTTCAC
Homo sapiens tumor protein p53 (p53)	NM_000546.5	GGCCCACTTCACCGTACTAA	GTGGTTTCAAGGCCAGATGT
Homo sapiens RB transcriptional corepressor 1 (RB1)	NM_000321.2	TGCATGGCTCTCAGATTCAC	AGTTGGTCCTTCTCGGTCCT
Homo sapiens E2F transcription factor 1 (E2F1)	NM_005225.3	TGTGCATGAGTCCATGTGTG	GGCCGAAAGTGCAGTTAGAG
Simian virus 40 (SV40)	NC_001669.1	TGGGGAGAAGAACATGGAAG	AAATGAGCCTTGGGACTGTG
Homo sapiens telomerase reverse transcriptase (hTERT)	NM_198253.3	GCTAGTGGACCCCGAAGG	CCTCCCTGACGCTATGGTT