Immortalizing Mesenchymal Stromal Cells from Aged Donors While Keeping Their Essential Features

Table 4

Flow cytometric analysis of mesenchymal markers CD29, CD44, CD73, CD90, and CD105 and the hematopoietic markers CD34 and CD45; 10⁵ events were acquired for each sample.

Cells	Passage	CD29	CD44	CD73	CD90	CD105	CD34	CD45
MSC#6	4	99.2%	99.6%	98.4%	96.2%	85.2%	9.9%	0.1%
T-MSC#6	(4 + 3)	99.0%	98.9%	96.2%	99.1%	82.3%	1.5%	0.4%
iMSC#6	(4 + 9 + 6)	99.2%	98.9%	98.0%	98.3%	81.0%	0.1%	0.0%
iMSC#6 ()	(4 + 9 + 44)	98.2%	98.9%	97.1%	99.5%	73.8%	0.0%	0.0%
MSC#8	4	98.6%	99.4%	98.3%	98.9%	91.6%	9.8%	0.0%
T-MSC#8	(4 + 4)	93.9%	92.7%	86.3%	95.0%	46.0%	0.4%	0.1%
iMSC#8	(4 + 2 + 15)	99.9%	99.6%	99.6%	99.0%	42.1%	0.0%	0.1%
iMSC#8 ()	(4 + 2 + 32)	98.8%	98.8%	98.7%	99.1%	38.4%	0.4%	0.2%
MSC#9	4	96.3%	97.0%	95.5%	96.2%	42.2%	0.6%	0.3%
T-MSC#9	(4 + 4)	98.4%	97.9%	69.6%	95.5%	71.7%	0.1%	0.2%
iMSC#9	(4 + 2 + 15)	98.4%	95.7%	97.3%	92.5%	37.3%	0.2%	0.4%
iMSC#9 ()	(4 + 2 + 35)	98.8%	98.4%	98.7%	99.3%	44.6%	0.9%	0.9%
MSC#10	4	97.9%	97.5%	93.0%	98.7%	76.0%	0.6%	0.3%
T-MSC#10	(4 + 2)	98.5%	98.1%	85.4%	97.4%	73.7%	0.0%	1.0%
iMSC#10	(4 + 3 + 14)	98.9%	98.9%	92.8%	99.1%	74.0%	0.4%	0.2%
iMSC#10 ()	(4 + 3 + 31)	98.4%	98.8%	93.9%	98.4%	82.4%	0.3%	2.7%
3a6	( + 30)	99.2%	99.8%	98.0%	98.3%	61.7%	0.1%	0.0%