Essentiality of CTNNB1 in Malignant Transformation of Human Embryonic Stem Cells under Long-Term Suboptimal Conditions

Figure 2

shRNA induces the downregulation of CTNNB1 expression in hESCs. (a) Western blot analysis of CTNNB1 protein in the shCTNNB1 group, empty vector group (MOCK), and uninfected control group (WT) hESCs. (b) Immunofluorescence assay of CTNNB1 and OCT4 in shRNAs, MOCK, and WT hESCs (). (c) Semiquantitative RT-PCR validation of mRNA expression levels of pluripotency genes in shCTNNB1, MOCK, and WT hESCs. Error bars denote from three independent experiments (, , , , relative to WT hESCs).

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