Shear Stress Triggers Angiogenesis of Late Endothelial Progenitor Cells via the PTEN/Akt/GTPCH/BH4 Pathway

Figure 6

PTEN/Akt and GTPCH/BH4 pathway blockade inhibits in vivo angiogenesis of EPCs treated with LSS. Representative photographs (a) and quantification analysis showing in vivo angiogenesis capacity of EPCs treated with GTPCH I knockdown and Akt inhibition for improving blood perfusion in ischemic hind limb (b, c) ( vs. medium, ; ^# vs. EPCs under static condition group, ; ^& vs. LSS+EPC group, per group). Least significant difference was applied for the post hoc test in statistical analysis. Concentration of LY 294002: 10 μmol/l. (d) Representative photographs and quantification analysis showing in vivo angiogenesis capacity of EPCs by CD31 staining ( vs. medium, ; ^# vs. EPCs under static condition group, ; ^& vs. LSS+EPC group, per group). Scale bar 50 μm. Concentration of LY 294002: 10 μmol/l. LSS = laminar shear stress; hpf = high power field.

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