Human Supernumerary Teeth-Derived Apical Papillary Stem Cells Possess Preferable Characteristics and Efficacy on Hepatic Fibrosis in Mice

Figure 1

Isolation and phenotype of SCAP-Ss and DPSCs. (a) Age distribution of the patients with supernumerary teeth during the years of 2016–2018 at the dental clinic of the First Affiliated Hospital of Fujian Medical University. (b) The periapical radiography of the supernumerary teeth (mesiodens) and permanent teeth of the patients in this study. (c) The removed supernumerary tooth and permanent tooth in a 10 cm dish. (d) Phase contrast images of primary (upper panel) and passages (bottom panel) of SCAP-Ss and DPSCs derived from the supernumerary tooth and permanent tooth (P1, P3), respectively. μm (upper panel) or μm (bottom panel). (e) Flow cytometry (FCM) analysis for surface markers (CD73, CD90, CD105, CD44, CD31, CD34, CD45, and HLA-DR) of SCAP-Ss and DPSCs in DMEM/F12 basal medium supplemented with 1% NEAA (Gibco), 1% L-glutamine (Gibco), 1% Penicillin and streptomycin (ThermoFisher), 10% fetal bovine serum, 4 ng/ml EGF, and 4 ng/ml bFGF. (f) Statistical analysis of FCM data in (e). All data were shown as the (). NS: not significant.

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