Human Supernumerary Teeth-Derived Apical Papillary Stem Cells Possess Preferable Characteristics and Efficacy on Hepatic Fibrosis in Mice

Figure 5

Preclinical therapeutic effect of SCAP-Ss and DPSCs on mice with hepatic fibrosis. (a) Schematic of the process of inducing the hepatic fibrosis model and the efficacy of SCAP-Ss and DPSCs on mice. (b) Histopathologic analysis of liver tissues of mice (NC, CCl₄, CCl₄+SCAP-Ss, and CCl₄+DPSCs) at week 12 by hematoxylin and eosin (H&E) staining. NC: negative control. μm. (c) Histopathologic analysis of the indicated liver tissues of mice at week 12 by Masson staining. μm. (d) Indicators of liver function including alanine aminotransferase (ALT) and aspartic transaminase (AST) in the indicated mice at week 12. Briefly, the serum was enriched into a 1.5 ml microanticoagulant tube from peripheral blood of mice by centrifugation at for 5 min in the indicated groups (NC, CCl₄, CCl₄+SCAP-Ss, and CCl₄+DPSCs). Then, the ALT value and AST value in the serum were qualified with the commercial automatic biochemical analyser according to the instructions. All data were shown as the (). (e) qRT-PCR analysis of the liver reconstruction-associated genes (Ck-18, Ck-19, and Hgf) in the indicated mice at week 12 as described in Materials and Methods. All data were shown as the (). ; NS: not significant. All values are normalized to the NC group (=1).

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