iRoot SP Promotes Osteo/Odontogenesis of Bone Marrow Mesenchymal Stem Cells via Activation of NF-κB and MAPK Signaling Pathways

Figure 2

Impact of SP on osteo/odontogenic differentiation of BMSCs. (a) Western blot was used to investigate DSPP, OPN, RUNX2, and OSX protein expression at days 0, 3, and 7. Gray scale inspection of protein bands by ImageJ. , and . (b) RT-PCR demonstrated the relative gene expression in the control group, as well as the SP group, at days 0, 3, and 7. , and . (c) ALP staining and ALP activity for the control group, SP group, MM group, and SP group at day 7. . . (d) Figures of Alizarin Red staining and CPC assay in different groups. . . (e) Immunofluorescence staining of ALP and RUNX2 in SP-treated BMSCs.

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