iRoot SP Promotes Osteo/Odontogenesis of Bone Marrow Mesenchymal Stem Cells via Activation of NF-κB and MAPK Signaling Pathways

Figure 3

Impact of iRoot SP on the NF-κB cascade of BMSCs. (a) Protein expression of IκBα, p-IκBα, P65, and p-P65 in the cytoplasm and nuclear P65 of SP-treated BMSCs at different time points. GAPDH was used as the internal control. Gray scale evaluations of protein by ImageJ. , , . (b) Immunofluorescence assay of nuclear P65 at different time points. . (c) Protein titers of cytoplasm P65, p-P65, IκBα, and p-IκBα, as well as nuclear P65, in the NC group, SP group, and SP + BMS345541 group at 30 min. GAPDH and Histone 3 were used as internal controls. Gray scale assessment of protein bands by ImageJ. , and . (d) Gene expression of Dspp, Opn, Runx2, and Osx in the NC group, SP group, and SP + BMS345541 group at 30 min. , . (e) Protein expression of DSPP, OPN, RUNX2, and OSX in different groups at day 7. , , . (f) ALP staining and ALP enzyme activity in the BMS345541 + SP group and SP group after 5 days. . , and . (g) Figures of Alizarin Red S staining of BMSCs in different groups. Absorbance was measured to evaluate the degree of mineralization. . , and .

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