iRoot SP Promotes Osteo/Odontogenesis of Bone Marrow Mesenchymal Stem Cells via Activation of NF-κB and MAPK Signaling Pathways

Figure 4

iRoot SP activated the MAPK cascades of BMSCs. (a) Protein titers of JNK, phospho-JNK, P38, p-p38, ERK, and p-ERK of SP-treated BMSCs and quantitative analysis of the ratios at different time points. , and . (b) The expression of MAPK-related proteins treated with cascade repressors (SP: SP600125, SB: SB203580, and U: U0126) was investigated by Western blot. , and . (c) Immunofluorescence staining of P-JNK, p-p38, and P-ERK after suppression of the MAPK cascades. . (d) Western blot assessment of osteo/odontogenic proteins in the different groups at day 7. Quantitative analysis of protein bands was performed using ImageJ. , , and . (e) MRNA expression of osteo/odontogenic differentiation-associated genes was quantified by RT-PCR. , , and . (f) ALP staining and quantification of ALP enzyme activity of BMSCs cultured with complete medium, SP, SP + SB203580, SP + SP600125, and SP + U0126 for 5 days. . , and . (g) Alizarin Red S staining and quantification analysis of calcium nodules in the different groups at different time points. . , and .

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