Cathepsin K Deficiency Impaired Ischemia-Induced Neovascularization in Aged Mice

<div>CatK<sup>−/−</sup> impairs bone marrow-derived CD31<sup>+</sup>c-Kit<sup>+</sup> EPC-like cell mobilization. (a, b) Representative flow cytometry plots and quantitative analysis data show the numbers of CD31<sup>+</sup>c-Kit<sup>+</sup> cells in bone marrow (BM) and peripheral PB of both genotypes of old mice (counted total <svg height="11.9413pt" id="M16" style="vertical-align:-0.3499002pt" version="1.1" viewbox="-0.0498162 -11.5914 37.3068 11.9413" width="37.3068pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M412 140C382 77 369 73 315 73H129L270 222C362 320 402 379 402 466C402 571 322 635 234 635C177 635 130 609 99 576L42 495L64 475C90 514 133 568 201 568C274 568 318 519 318 435C318 349 255 267 193 193C144 135 87 78 32 23V0H405C417 45 427 89 440 131L412 140Z"></path></g><g transform="matrix(.013,0,0,-0.013,9.145,0)"><path d="M528 54L331 254L528 455L492 493L294 291L96 493L60 455L257 254L60 54L96 16L294 217L492 16L528 54Z"></path></g><g transform="matrix(.013,0,0,-0.013,19.682,0)"><path d="M384 0V27C293 34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g><g transform="matrix(.013,0,0,-0.013,25.922,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.0091,0,0,-0.0091,32.209,-5.741)"><path d="M462 177V227H365V632H320C217 496 116 350 21 208V177H284V109C284 43 280 38 190 31V0H451V31C369 38 365 43 365 108V177H462ZM284 227H88C155 336 218 430 282 519H284V227Z"></path></g></svg> MNCs) at day 7 after ischemic surgery. (c, d) Representative immunoblot images and combined quantitative data show that CatK<sup>−/−</sup> reduced the levels of c-Notch1 and p-Akt proteins in bone marrow-derived c-Kit<sup>+</sup> cells. 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ANOVA and Tukey’s post hoc tests.</div>

Stem Cells International

fig3

Figure 3

Figure 3: Cathepsin K Deficiency Impaired Ischemia-Induced Neovascularization in Aged Mice 