Size-Optimized Microspace Culture Facilitates Differentiation of Mouse Induced Pluripotent Stem Cells into Osteoid-Rich Bone Constructs

Figure 4

Microspace size influences the morphology and mineralization of OI-iPSC constructs. Representative images of histological analysis of OI-iPSC constructs in Elp400, Elp500, and Elp900. Paraffin-embedded sections (without decalcification) of OI-iPSC constructs at days 21, 28, and 35 were stained with von Kossa and counterstained with methylene blue. Black stain indicates mature mineralization. Dark blue and light blue indicate cells and ECM, respectively. Scale bars: 100 μm.