Surgical Injury and Ischemia Prime the Adipose Stromal Vascular Fraction and Increase Angiogenic Capacity in a Mouse Limb Ischemia Model

Figure 2

Canonical pathways and upstream-downstream effects involved in SVF priming. Gene expression profiles were compared between primed SVFs and nonprimed SVFs (i.e., 0 vs. 1 day or 0 vs. 3 days after priming), and the lists of differentially expressed genes were imported into Ingenuity Pathway Analysis software. (a) Canonical pathways were clustered according to the activation -score. The heat map indicates the -score based on alterations of “seed molecules” among the dataset. Each column indicates a repeat of experiments (observation) from a distinct time point (comparisons of 0 vs. 1 day and 0 vs. 3 days). The clustering overrepresented immune-related pathways, especially those of innate immunity (red underlines). Cell cycle-related pathways were overrepresented on day 3 after the priming (green underlines). (b) Lists of upstream regulators, the values (Fisher’s exact test) of which were the lowest. (c) Expected biological functions downstream of the gene expression changes are shown. Tiled boxes represent the functionally curated gene groups, the area of which is inversely correlated with the value (Fisher’s exact test). Small boxes are assembled in a larger box, creating a more common biological annotation. The color of each box indicates the activation -score, although the algorithm is different from that of canonical pathway analysis. Grey-shaded boxes indicate that it was infeasible to determine activation or inactivation. SVF: stromal vascular fraction.

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