Surgical Injury and Ischemia Prime the Adipose Stromal Vascular Fraction and Increase Angiogenic Capacity in a Mouse Limb Ischemia Model

Figure 5

A combination of surgical injury and ischemia was necessary for efficient blood flow recovery during limb ischemia. Blood flow recovery following injection of primed SVFs. (a) Representative laser Doppler blood perfusion imaging. Compared to the sham group (without SVF transplantation), the surgical injury-only group or the ischemia-only group did not show improved blood flow recovery on days 3, 7, and 14 after SVF injection. In contrast, efficient blood flow recovery was achieved in the injury+ischemia group. (b) Quantitative assessment of serial changes in blood perfusion. There was no significant difference in blood perfusion among the sham group, the injury-only group, and the ischemia-only group. The injury+ischemia group showed significantly higher blood perfusion than the sham group, the injury-only group, and the ischemia-only group on days 7 and 14 after injection. (c) Representative appearance of the ischemic (left) and nonischemic (right) hindlimbs. Note that the ischemic limb treated with both injury and ischemia retained an almost intact appearance. . SVF: stromal vascular fraction.

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