Isolation and Characterization of Human Synovial Fluid-Derived Mesenchymal Stromal Cells from Popliteal Cyst

Figure 3

(a) SF-MSC growth curve: cells showed normal exponential cell growth patterns with a steady increase in number during an 11-day culture period. The determination and calculation of the doubling time to assess proliferation (CCK-8) showed that the growth rate was slow during the first 3 days and rapid from 4 to 8 days. The cells reached the platform stage (stopped growing or entered apoptosis) at 9~11 days. These cells showed normal exponential cell growth patterns with a steady increase in number during an 11-day culture period, as well as general mesenchymal stem cell features. (b) Curve diagram of population doublings and doubling time with passages of rUSCs. (c) Cell cycle of SF-MSCs: the data showed that 94.36% of cells were in G0/G1 phase, 0.49% were in G2/M phase, and only 5.15% were in the S phase, indicating that the cells have strong proliferative abilities.

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