Isolation and Characterization of Human Synovial Fluid-Derived Mesenchymal Stromal Cells from Popliteal Cyst

Figure 7

Differentiation characteristics of SF-MSCs at passage 2. (a) Representative of early osteogenesis was detected by alkaline phosphatase staining at day 7. (b) Representative of osteogenesis detected as calcium deposition is an indication of osteogenesis and was detected using Alizarin Red at day 22. (c) Representative of adipogenic differentiation visualized by Oil Red O staining of the intracellular lipid vesicles at day 21. (d) Representative of chondrogenic differentiation detected by Alcian blue staining at day 28. Micrographs are representative of 12 experiments.

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