Phosphodiesterase 10A Is a Mediator of Osteogenic Differentiation and Mechanotransduction in Bone Marrow-Derived Mesenchymal Stromal Cells

Figure 6

Effect of PDE10A inhibition and cAMP stimulation on mechanotransduction in primary MSC. Relative mRNA expression of the mechanoresponsive genes PTGS2 and FOS 15 min after cyclic stretching and PDE10A 4 h after cyclic stretching. Cells were pretreated with 10 μM papaverine for 24 h or pretreated with 1 mM db-cAMP for 1 h. Results are shown as and normalized to the values obtained from stretched samples. Fold change was calculated with the ΔΔCT method, and RPS27A served as the housekeeping gene. Significances were calculated with the Student -test (; ). cs: cyclic stretching; db-cAMP: dibutyryl-cAMP; FOS: fos protooncogene; Papa: papaverine; PDE10A: phosphodiesterase 10A; PTGS2: prostaglandin-endoperoxide synthase 2.