Cerebrospinal Fluid Pulsation Stress Promotes the Angiogenesis of Tissue-Engineered Laminae

Figure 10

(a–c) Representative images of BMP-2 IHC staining of newborn laminae in the CSFP group and the Non-CSFP group in the 4^th, 8^th, and 12^th weeks. (g) The BMP-2 IHC staining of the paraspinal surface of the newborn laminae in the 12^th week. (h) The BMP-2 IHC staining of the dural surface of the newborn laminae in the 12^th week. (i) Quantification of BMP-2 expression by IHC staining. QRT-PCR analysis of Osterix (j) and ODG-3 (k) expressions. The red arrow showed positive staining. The dashed line in images (a), (b), and (c) showed the vertebral canal. The dashed line in images (d) and (e) showed the outer cortex of native laminae.