A Xeno-Free Strategy for Derivation of Human Umbilical Vein Endothelial Cells and Wharton’s Jelly Derived Mesenchymal Stromal Cells: A Feasibility Study toward Personal Cell and Vascular Based Therapy

<div>Vessel-like structure formation in 2-dimensional (2D) culture. After 3 days cultured in endothelial growth media, WJ-MSCs (a, b), HUVECs (c, d), autogenic (e, f), and allogenic (g, h) HUVECs/WJ-MSCs coculture were stained with antibodies directed against human CD31 (brown). Arrows indicated CD31 stained VLS in both of autogenic and allogenic HUVECs/WJ-MSCs coculture condition. Scale bar: 200 <i>μ</i>m (a, c, e, and g) and 100 <i>μ</i>m (b, d, f, and h).</div>

Stem Cells International

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Figure 4: A Xeno-Free Strategy for Derivation of Human Umbilical Vein Endothelial Cells and Wharton’s Jelly Derived Mesenchymal Stromal Cells: A Feasibility Study toward Personal Cell and Vascular Based Therapy 

Figure 4 | A Xeno-Free Strategy for Derivation of Human Umbilical Vein Endothelial Cells and Wharton’s Jelly Derived Mesenchymal Stromal Cells: A Feasibility Study toward Personal Cell and Vascular Based Therapy 