Effects of DMSO on the Pluripotency of Cultured Mouse Embryonic Stem Cells (mESCs)

Figure 2

DMSO does not affect apoptotic and cell cycle profiles in cultured mESCs. The percentage of apoptotic/necrotic cells was assessed via Annexin V and PI staining after 24 and 48 h culture in the presence/absence of DMSO. (a) Flow cytometry dot plot from necrosis/apoptosis detection at the 48 h time point. Four populations were identified: live cells (negative for both Annexin V and PI), early apoptotic cells (positive for Annexin V and negative for PI), late apoptotic cells (positive for both Annexin V and PI), and dead cells (only positive for PI). (b) Percentage of cells in the defined populations at the 24 h time point. (c) Percentage of cells in the defined populations at the 48 h time point. Four independent experiments were conducted. (d) Flow cytometry histograms obtained from the analysis of cell cycle using PI staining at the 48 h time point. (e) Percentage of cells in each cell cycle phase at the 24 h time point. (f) Percentage of cells in each cell cycle phase at the 48 h time point. At least five independent experiments were performed. Results are expressed as . Statistical significance was considered when .

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