In Vitro Oxygen-Glucose Deprivation-Induced Stroke Models with Human Neuroblastoma Cell- and Induced Pluripotent Stem Cell-Derived Neurons

Figure 3

Cell viability and cytotoxicity of SH-SY5Y- and hiPSC-neurons after OGD and ASC coculture. (a, e) Representative images of live/dead staining after 72 h of reperfusion in SH-SY5Y-neurons (a) and hiPSC-neurons (e). Green = live cells/calcein-AM; red = dead cells/EthD-1. Scale bar, 100 μm. (b) Area of live SH-SY5Y-neurons and (f) hiPSC-neurons. (c–g) Area of dead SH-SY5Y-neurons (c) and (g) hiPSC-neurons. For quantification of live and dead cells, images were analyzed from two to three wells/group, and four images were taken from each well (one experiment for SH-SY5Y-neurons and representative data of two separate experiments for hiPSC-neurons). (d) Analysis (CyQuant) of the number of SH-SY5Y-neurons (/group, data from four independent experiments) and (h) hiPSC-neurons (/group, representative data of two separate experiments). All statistical analyses were performed using an independent -test, statistical significance: , , and . All quantitative data are presented as the .

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