Quantification and Comprehensive Analysis of Mesenchymal Stromal Cells in Bone Marrow Samples from Sickle Cell Disease Patients with Osteonecrosis

Figure 4

BM-MSCs from SCD and NS patients display similar immunophenotypes and in vitro characteristics. (a) Morphology and cell growth expansion of BM-MSC. Cells were subcultured and counted on the indicated day for 7 days. (b) Immunocytochemistry detection shows α-SMA and vimentin-positive BM-MSCs. Nuclei were stained with Hoechst dye (blue). (c) Flow cytometry histograms for positive MSC specific markers (purple line) and the respective isotype-matched control (green line) are shown. (d) Multipotential BM-MSCs from representative SCD and NS patients were differentiated toward adipogenic, osteoblastic, and chondrogenic lineages. Accumulation of intracellular lipid vacuoles shown by Oil Red O staining (left), calcium-rich extracellular matrix as evidenced by Alizarin red S (middle), and static micromass cell culture stained with Alcian blue (right). Statistical analyses (Student -test) were performed between values, and the data are reported as the mean and standard deviation.  μm.

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