Quantification and Comprehensive Analysis of Mesenchymal Stromal Cells in Bone Marrow Samples from Sickle Cell Disease Patients with Osteonecrosis

Figure 5

BM-MSCs from SCD and NS patients showed no significant differences in migration, osteogenic marker, and secreted cytokines. (a) Boyden chamber analysis. Uncoated Boyden chambers were used to analyze migration of BM-MSCs. Cells that migrated through the chambers were stained with crystal violet and quantitated (right). (b) Quantification of intracellular osteocalcin expression in BM-MSCs grown under osteoinductive conditions for 10 days. (c) Enzyme-linked immunosorbent assay for interleukin-8 (IL-8), stromal cell-derived factor-1 alpha (SDF-1α), and transforming growth factor-beta (TGF-β). Concentration (pg/mL) of cytokines and growth factors in supernatants of BM-MSCs. Statistical analyses (Mann–Whitney test) were performed between values, and the data are reported as the median and interquartile range.

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