Stem Cells International / 2020 / Article / Fig 4

Research Article

Myocardin-Related Transcription Factor A (MRTF-A) Regulates the Balance between Adipogenesis and Osteogenesis of Human Adipose Stem Cells

Figure 4

Late osteogenesis of MRTF-A inhibitor-treated hASCs. Matrix mineralization of hASCs was analyzed with AR staining after 21 d of culture with CCG-1423 (a) or CCG-100602 (b) inhibitors. Quantitative results of AR staining are presented as graphs, and corresponding representative images of the stained wells (area 1.9 cm2) are displayed below the columns, bright red dye represents mineral. , independent biological replicates from 3 donors. Significance level 5%. (c) Representative OPN-stained samples of MTRF-A inhibitor-treated hASCs at 21 d. The cells were imaged with a fluorescence microscope using Alexa 488 for OPN (green), Alexa 546 for actin (red), and DAPI (blue) filters, and 20x magnification. Scale bar 100 μm, same scale in every image. BM: basic medium; OM: osteogenic medium.
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