Myocardin-Related Transcription Factor A (MRTF-A) Regulates the Balance between Adipogenesis and Osteogenesis of Human Adipose Stem Cells

Figure 4

Late osteogenesis of MRTF-A inhibitor-treated hASCs. Matrix mineralization of hASCs was analyzed with AR staining after 21 d of culture with CCG-1423 (a) or CCG-100602 (b) inhibitors. Quantitative results of AR staining are presented as graphs, and corresponding representative images of the stained wells (area 1.9 cm²) are displayed below the columns, bright red dye represents mineral. , independent biological replicates from 3 donors. Significance level 5%. (c) Representative OPN-stained samples of MTRF-A inhibitor-treated hASCs at 21 d. The cells were imaged with a fluorescence microscope using Alexa 488 for OPN (green), Alexa 546 for actin (red), and DAPI (blue) filters, and 20x magnification. Scale bar 100 μm, same scale in every image. BM: basic medium; OM: osteogenic medium.

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