Myocardin-Related Transcription Factor A (MRTF-A) Regulates the Balance between Adipogenesis and Osteogenesis of Human Adipose Stem Cells

Figure 5

Intracellular protein levels of MRTF-A, β-actin, α-SMA, pMLC, and MLC as a response to MRTF-A inhibition. hASCs were cultured 7 d in BM, OM, or AM media supplemented with 20 μM CCG-1423 or 12 μM CCG-100602. (a) Representative WB results of immunoblotted MRTF-A, β-actin, α-SMA, pMLC, and MLC. Semiquantitative results of MRTF-A (b), α-SMA (c), pMLC (d), and MLC (e) normalized with β-actin. MRTF-A and α-SMA: independent experiments, 2 donors; pMLC and MLC: independent experiments, 2 donors. BM: basic medium; OM: osteogenic medium; AM: adipogenic medium.

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