Myocardin-Related Transcription Factor A (MRTF-A) Regulates the Balance between Adipogenesis and Osteogenesis of Human Adipose Stem Cells

Table 1

Reagents used in cytochemical staining.

Antibody type	Antibody	Host species	Dilution	Incubation
Primary	Anti-Collagen I (ab90395)^1	Mouse	1 : 2000	+4°C, overnight
Primary	Anti-Osteopontin (ab8448)^1	Rabbit	1 : 100	+4°C, overnight
Primary	Anti-Perilipin-1 (ab3526)^1	Rabbit	1 : 600	+4°C, overnight
Secondary	Anti-mouse IgG Alexa fluor 488 (A11029)^2	Goat	1 : 500	+4°C, 45 min
Secondary	Anti-rabbit IgG Alexa fluor 488 (A21206)^2	Donkey	1 : 500	+4°C, 45 min
—	Phalloidin–Tetramethylrhodamine B isothiocyanate (TRITC)^3	—	1 : 500	+4°C, 45 min
—	DAPI^3	—	1 : 2000	RT, 5 min

¹Abcam, Cambridge, United Kingdom. ²Thermo Fisher Scientific. ³Sigma-Aldrich.