Research Article
Myocardin-Related Transcription Factor A (MRTF-A) Regulates the Balance between Adipogenesis and Osteogenesis of Human Adipose Stem Cells
Table 2
Primary and secondary antibodies used in immunodetection.
| | Antibody type | Antibody | Host species | Dilution | Incubation |
| | Primary | Anti-MKL1/MRTF-A (14760S)1 | Rabbit | 1 : 800 | +4°C, overnight | | Primary | Anti-MLC (3672S)1 | Rabbit | 1 : 500 | +4°C, overnight | | Primary | Anti-pMLC (3671S)1 | Rabbit | 1 : 500 | +4°C, overnight | | Primary | anti-α-SMA (14968S)1 | Rabbit | 1 : 1000 | +4°C, overnight | | Primary | Anti-β-actin (sc47778)2 | Mouse | 1 : 2000 | RT, 1 h | | Secondary | Anti-rabbit IgG-HRP (7074S)1 | Goat | 1 : 2000 | RT, 1 h | | Secondary | Anti-mouse IgG-HRP (sc2005)2 | Goat | 1 : 2000 | RT, 1 h |
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1Cell Signaling Technology, Danvers, Massachusetts, USA. 2Santa Cruz Biotechnology, Dallas, Texas, USA.
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