Iron Overload Impairs Bone Marrow Mesenchymal Stromal Cells from Higher-Risk MDS Patients by Regulating the ROS-Related Wnt/β-Catenin Pathway

Table 2

Gene-specific primers used in RT-PCR.

Genes	Primer sequence (5–3)
VEGFA	Forward 5AGCCTTGCCGCCTTGCTGCTCTA3; Reverse 5GTGCTGGCCTTGGTGAGG3
CXCL12	Forward 5GAGCTACAGATGCCCATGC3; Reverse 5CTTTAGCTTCGGGTCAATGC3
TGF-β1	Forward 5GCGTGCTAATGGTGGAAACC3; Reverse 5GCTTCTCGGAGCTCTGATGTG3
Caspase3	Forward 5TTGTAGAAGTCTAACTGGAA3; Reverse 5CCATGTCATCATCAACAC3
β-Catenin	Forward 5GCAGCTGCTGTTTTGTTCCG3; Reverse 5GATTGCTGTCACCTGGAGGC3
β-Actin	Forward 5TGGACATCCGCAAAGACCTGT3; Reverse 5CACACGGAGTACTTGCGCTCA3