Gene Profiles in the Early Stage of Neuronal Differentiation of Mouse Bone Marrow Stromal Cells Induced by Basic Fibroblast Growth Factor

Figure 1

Characterization of BMSCs. (a) BMSCs from initial isolation to passage 50. Cell morphology became relatively homogeneous from passage 6 onwards and maintained the appearances till passage 50 or later. (b) Fluorescent-activated cell sorting (FACS) analysis for cell surface markers. Throughout the passages, markers for mesenchymal stem cells, Sca-1 and CD44, remained highly elevated and slightly decreased with passage number. The expressions for CD90.1, CD45, CD117, and CD34 were negligible throughout thus excluding contamination of hematopoietic stem cells from the isolated culture. (c) FACS analysis for CD105 and CD45. The expression of CD105 was positive, and CD45 was negligible.

(a)

(b)

(c)