Generation of Inducible CRISPRi and CRISPRa Human Stromal/Stem Cell Lines for Controlled Target Gene Transcription during Lineage Differentiation

<div>Comparison of the efficiency of gene inhibition or gene activation in control hMSC, inducible CRISPRi hMSC, and CRISPRa hMSC. (a, b) Control (Ctrl) hMSC and inducible CRISPRi were transfected with siRNA-Ctrl, siRNA-ALP, Ctrl gRNA, or ALP gRNA oligo and for 2 or 7 days as described in Methods. ALP expression was measured by RT-qPCR at day 2 (a) or day 7 (b). (c, d) Control (Ctrl) hMSC and inducible CRISPRa were transfected with pcDNA3 vector plasmid, pcDNA3-ALP plasmid by electroporation, or Ctrl gRNA and ALP gRNA oligo by DharmaFECT™ Transfection Reagent as described in Methods. ALP expression was measured by RT-qPCR at day 2 (c) and day 7 (d). 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Stem Cells International

fig4

Figure 4

Figure 4: Generation of Inducible CRISPRi and CRISPRa Human Stromal/Stem Cell Lines for Controlled Target Gene Transcription during Lineage Differentiation 

Figure 4 | Generation of Inducible CRISPRi and CRISPRa Human Stromal/Stem Cell Lines for Controlled Target Gene Transcription during Lineage Differentiation 