Generation of Inducible CRISPRi and CRISPRa Human Stromal/Stem Cell Lines for Controlled Target Gene Transcription during Lineage Differentiation

<div>The technical flow chart of inducible inhibition or activation-specific genes in CRISPRi or CRISPRa human stromal/stem cells (hMSCs). Human bone marrow stromal/stem cell line (hMSC-TERT) stable transfected with dCas9 TRE-KRAB or dCas9TRE-VP64 to establish the inducible CRISPRi or CRISPRa cell lines. In these cells, with simply transfecting the small molecular guide RNAs (gRNAs) that target different specific genes, it can easily inhibit or activate specific gene expressions with or without doxycycline (Dox) in cell culturing mediums or differentiation induction medium.</div>

Stem Cells International

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Figure 5

Figure 5: Generation of Inducible CRISPRi and CRISPRa Human Stromal/Stem Cell Lines for Controlled Target Gene Transcription during Lineage Differentiation 

Figure 5 | Generation of Inducible CRISPRi and CRISPRa Human Stromal/Stem Cell Lines for Controlled Target Gene Transcription during Lineage Differentiation 