Hypoxia-Preconditioned Wharton’s Jelly-Derived Mesenchymal Stem Cells Mitigate Stress-Induced Apoptosis and Ameliorate Human Islet Survival and Function in Direct Contact Coculture System

Table 1

The designed primers.

Gene	Product (bp) size	Sequence	Annealing temperature (°C)
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH)	127	F: GCTCATTTCCTGGTATGACAACG	61
		R: CTCTCTTCCTCTTGTGCTCTTG
B cell lymphoma 2 (Bcl-2)	105	F: GATGGGATCGTTGCCTTATGC	62
		R: CAGTCTACTTCCTCTGTGATGTTGT
BCL-2-associated X protein (Bax)	134	F: TTCTGACGGCAACTTCAACT	60
		R: GGAGGAAGTCCAATGTCCAG
Insulin	150	F: CTTCTACACACCCAAGACCC	60
		R: CTGGTACAGCATTGTTCCAC
Vascular endothelial growth factor (VEGF)	111	F: CTTCAAGCCATCCTGTGTGC	58
		R: ATCCGCATAATCTGCATGGTG
Hypoxia-inducible factor 1-alpha (HIF-1α)	131	F: GCAGCAACGACACAGAAACT	60
		R: TTCAGCGGTGGGTAATGGAG
Caspase-3	148	F: ACTCCACAGCACCTGGTTATT	61
		R: TCTGTTGCCACCTTTCGGTT