Low-Intensity Pulsed Ultrasound Modulates RhoA/ROCK Signaling of Rat Mandibular Bone Marrow Mesenchymal Stem Cells to Rescue Their Damaged Cytoskeletal Organization and Cell Biological Function Induced by Radiation

Figure 4

LIPUS induced cytoskeleton remodeling and vinculin redistribution after irradiation via a RhoA/ROCK signaling pathway. The cells were divided into four groups: the control group (d) that received no treatments; the radiation group (e) that was irradiated with 4 Gy of X-ray for 12 h; the LIPUS group (f) that was exposed to 2 h of LIPUS treatment after irradiation for 12 h; and the inhibitor group (g) that was subjected to Y-27632 30 min before the LIPUS treatment. Then, the cells were immunostained with F-actin (D1, E1, F1, and G1) and counter stained with TRITC-vinculin (D2, E2, F2, and G2) and DAPI (D3, E3, F3, and G3). Graphs D4, E4, F4, and G4 are the merged images. The white arrowheads represent the changes in the cytoskeleton. The yellow arrowheads represent the changes in the vinculin distribution. Scale bars 50 μm.

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