Stem Cells International

Review Article

Dental Tissue-Derived Human Mesenchymal Stem Cells and Their Potential in Therapeutic Application

Table 2

Current clinical studies on treatment of dental diseases based on dental MSCs.


Application	Source of dental MSCs	Size	Therapeutic strategy			Delivery approach	Follow-up time	Outcome	Reference
Application	Source of dental MSCs	Size	Cells	Scaffolds	Growth factor	Delivery approach	Follow-up time	Outcome	Reference

Irreversible pulpitis	Mobilized hDPSCs from 3rd molar	5	Passage 7	—	G-CSF 300 ng	Autologous transplantation	1, 2, 4, 12, 24, 28, and 32 weeks	No adverse events EPT: positive response at 4 weeks MRI: SI in the root canal approached that of the normal pulp in untreated controls after 24 weeks CBCT: lateral dentin formation in three cases at 28 weeks	[37]
Dental trauma with pulp necrosis	hDPSCs from deciduous canine teeth	26	Two hDPSC aggregates containing /ml	—	Extracellular matrix	Autologous implantation	1, 3, 6, 9, 12, and 24 months	No significant side effects after 12 months Digital RVG: continued root development EPT: decrease in sensation thresholds CBCT: apical foramen width decreased, the length of the treated tooth root increased Laser Doppler flowmetry: increase in vascular formation Histology staining: regeneration of 3D whole dental pulp tissue	[79]
Irreversible pulpitis	hDPSCs from inflamed pulp	1	/ml	Membrane of collagen	Leukocyte platelet-rich fibrin (L-PRF)	Autologous implantation	6 and 36 months	Remaining free of any symptoms Periapical radiographs: a normal periapical area CBCT: intact periapical bone structures Sensitivity: delayed response to cold EPT: responsive Laser Doppler flowmetry: low blood perfusion	[80]
Periodontal intrabony defects	hDPSCs	11	Micrograft containing cells	Collagen sponge	—	Autologous implantation	6 and 12 months	No adverse events Clinical measurements: PD reduction, CAL increased, pocket closure, gingival improvement Radiographs: intrabony defect decreased	[90]
Periodontal intrabony defects	hDPSCs	15	Micrograft containing cells	Collagen sponge	—	Autologous implantation	6 and 12 months	No adverse events. Clinical measurements: PD reduction, CAL gain Radiographs: bone defect fill	[91]
Periodontal disease	hDPSCs	1	/ml	Collagen sponge	—	Allogeneic grafting	3 and 6 months	No signs or symptoms of rejection. Clinical evaluation: tooth mobility, periodontal pocket depth decreased CBCT: bone defect area significantly reduced	[92]

G-CSF: granulocyte colony-stimulating factor; EPT: electric pulp vitality testing; CBCT: cone beam computed tomography; SI: signal intensity; RVG: radiovisiography; PD: probing depth; CAL: clinical attachment level; PPD: probing pocket depth; BOP: bleeding on probing.