Enhanced Expression of ABCB1 and Nrf2 in CD133-Positive Cancer Stem Cells Associates with Doxorubicin Resistance

Figure 6

The effect of silencing CD133 expression on DXR resistance of CD44⁺CD133⁺ cells. (a) Representative histograms of flow cytometry analysis on the expression of CD133 in CD44⁺CD133⁺ cells after silencing by different dosages of targeted siRNA. Quantitative data in the histograms are presented as the mean fluorescent intensity from three independent experiments. (b) MTT assay was done to evaluate the cytotoxicity to DXR. Cells were treated with 5 nM siRNA for 48 hr followed by DXR treatment for another 48 hr. Data are expressed as a percent of baseline (before DXR treatment) from three independent experiments. vs. CD44⁺CD133^- cells. (c) Representative histograms of flow cytometry analysis on the expression of ABCB1 in cells after silencing by different dosages of targeted siRNA. Quantitative data in the histograms are presented as the mean fluorescent intensity from three independent experiments. (d) Representative histograms of flow cytometry analysis on the accumulation of DXR. Quantitative data in the histograms are presented as the mean fluorescent intensity from three independent experiments.

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