Regulation of CXCR6 Expression on Adipocytes and Osteoblasts Differentiated from Human Adipose Tissue-Derived Mesenchymal Stem Cells

Figure 2

CXCR6 knockdown by small hairpin RNA technique. (a) Quantitative CXCR6 gene expression and (b, c) protein intensity of CXCR6. trial per samples and control. The effect of CXCR6 knockdown on the capacity of hADMSC differentiation into adipogenic and osteogenic lineages by (d) ORO and ARS staining after 18 days of adipogenic and osteogenic induction. Magnification (×200). (e, f) Stained lipids and osteoblasts were dissolved and quantified. trial per samples and control. (g, h) Gene expression levels of adipogenic markers (PPARγ, FABP4, and C/EBPα) and osteogenic markers (ALP, RUNX2, and OCN) on Ad and Os transfected with sh-control and sh-CXCR6. trial per samples and control. , , and indicate statistically significant compared with the sh-control group.

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