Regulation of CXCR6 Expression on Adipocytes and Osteoblasts Differentiated from Human Adipose Tissue-Derived Mesenchymal Stem Cells

Figure 7

Level of CXCL16 secretion and CXCR6 expression by inhibition of ADAM10 activity in hADMSCs and differentiated adipocytes or osteoblasts. (a) Expression of transmembrane-bound CXCL16 in cells treated with ADAM10 inhibitor (GI254023x; 20 μM) was evaluated by ICC. Cells were stained with CXCL16 (green), and nuclei were stained with DAPI (blue). . (b) Quantification of fluorescent intensities of CXCL16 was performed by the ImageJ program. trial per samples and control. (c) CXCR6 expression on the surface of cells treated with GI254023x was evaluated by ICC. Cells were stained with CXCR6 (green), and nuclei were stained with DAPI (blue). . (d) Fluorescent intensities of CXCR6 were quantified by the ImageJ program. trial per samples and control. and indicate statistically significant compared with the hADMSC group. ^## and ^### indicate statistically significant between the NT- and GI254023x-treated groups in Ad or Os. ns: not significant.

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