The Paracrine Effect of Adipose-Derived Stem Cells Orchestrates Competition between Different Damaged Dermal Fibroblasts to Repair UVB-Induced Skin Aging

Figure 1

Assessment of the functions of HDFs cocultured with ASCs. (a) Schematic illustration of the cell experiment. (b) Representative images of senescence-associated β-galactosidase (SA-β-gal) staining. Positive blue staining of SA-β-gal appeared in senescent HDFs. (c) The average percentage of SA-β-gal-positive cells was quantified. Five random fields were selected for counting. (d) Proliferation of HDFs was assessed by CCK-8 assays. (e) HDF apoptosis was measured by FACS analysis after Annexin V-FITC/PI staining. (f) The apoptosis rates of HDFs were quantified. (g) Representative images of migrated HDFs (magnification, ×200). (h) The number of migrated HDFs per field. Five random fields were selected for counting. All experiments were independently conducted in triplicate, and the values are expressed as the . (, , ).

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