The Paracrine Effect of Adipose-Derived Stem Cells Orchestrates Competition between Different Damaged Dermal Fibroblasts to Repair UVB-Induced Skin Aging

Figure 4

Assessment of oxidative stress in HDFs cocultured with ASCs. (a) Flow cytometry showing the reactive oxygen species (ROS) levels in HDFs. (b) Quantification of ROS-positive cells. (c) Western blots showing Nrf2 and IL-6 expression in HDFs. (d) Western blots quantification of Nrf2 and IL-6. (e) RT-PCR analysis of Nrf2 and IL-6 gene expression in HDFs. (f) ELISA results showing the expression of IL-6 in the cell culture supernatant.

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