Galectin-3 Secreted by Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Reduces Aberrant Tau Phosphorylation in an Alzheimer Disease Model

Figure 1

Administration of hUCB-MSCs ameliorates behavioral dysfunction in 5xFAD mice. (a) The schedule of repeated injection (3 times) of hUCB-MSCs via the lateral ventricle of 5xFAD mice, which express human APP and PSEN1 transgenes with a total of five AD-linked mutations. After 4 weeks at the last injection, mice were subjected to behavioral tests, and the brains were collected to analyze tau phosphorylation with western blotting or IF. (b) The brains injected with hUCB-MSCs were stained with antihuman mitochondria (red). Fluorescence signals were observed in the cortex, DG, CPu, hypothalamus, and SVZ ( μm). (c) Various behavioral tests for hUCB-MSCs-injected 5xFAD mice (open field: resting duration, the distance in center, and number of rears; T-maze: alternation; per group; , ). CTRL: PBS-administrated 5xFAD; MSC: hUCB-MSC-administered 5xFAD.

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