Galectin-3 Secreted by Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Reduces Aberrant Tau Phosphorylation in an Alzheimer Disease Model

Figure 5

GAL-3 attenuates tau pathology in 5xFAD mice. (a) Schematics of human recombinant GAL-3 injection into the hippocampi of 5xFAD mice and the schedule of animal sacrifice. (b) After 1 week, GAL-3-injected 5xFAD mouse brains were extracted and assessed by western blotting with various phosphorylated tau antibodies. β-Actin was used as a loading control. Western blotting was analyzed using densitometric quantification ( per group, , ). (c) Sections of the cortical and DG regions were stained with specific antibodies for phosphorylated tau (red), tyr181, or ser404 ( μm). (d) GAL-3-injected or hUCB-MSC-administrated 5xFAD mouse brains were analyzed by western blotting with tyrosine 216 phosphorylated GSK-3β or total GSK-3β antibodies. β-Actin was used as a loading control. (e) Densitometric quantification of western blotting in (d) (, , per group or  ~ 7 per group). CTRL: PBS-administrated 5xFAD; GAL-3: GAL-3-injected 5xFAD; MSC: hUCB-MSC-administrated 5xFAD; DG: dentate gyrus; CA3: cornu ammonis 3.

(a)

(b)

(c)

(d)

(e)